Are you a professional or curious about the world of fungi - Gribb liquid culture is for you! We have a wide selection of gourmet and functional exotic mushrooms to choose from. Our mycelium culture is done in liquid culture or colonized cereal (spawn).
At Gribb our team prepares and tests all cultures ensuring the purity of the species and being free from contamination.
We advise you to study and gather knowledge on how to properly inoculate the substrate.
Contamination is easy to occur when growing mushrooms because the perfect environment for mushrooms to grow is also the perfect environment for fungus to grow. Therefore, remember to always sterilize everything and, if possible, carry out all inoculations under the flow of a HEPPA filter.
- Liquid Culture
- Ethyl alcohol or other disinfectant
- Disposable gloves and face mask
- Scissors or x-zato
- Impulse sealer (hot)
- Cereal BIO - get your CerealLoving™ here
- HEPPA filter (Flow Hood) - we build to measure, get in touch
Don't forget that we are dealing with living fungi and the worst competitor or enemy of a fungus...is another fungus.
Carrying out the following steps in a clean, sterile environment will influence the success of any and all inoculations.
HEPPA filter (Flow Hood)
We recommend that you carry out all your inoculations in a clean room free of contaminating agents and preferably in front of a HEPPA filter (flow hood) to minimize the risk of contaminants entering your culture.
Inoculate sterilized GrainLoving™ Cereal with Liquid Culture
Step 1 - Disinfect
Use sterile technique. We recommend that you wear gloves and a mask, clean hands and forearms with disinfectant and clothes that have not been in contact with sources of contamination.
Disinfect the inoculation area, as well as disinfect the sterilized cereal package.
Step 2 - Insert the Needle
Then, open the liquid culture package, remove the tip cap from the syringe and replace it with the 18G needle that follows with the culture.
Do this step quickly. Once the needle is in place, it's time to inoculate.
Ideally, this step should be performed in front of a HEPPA filter for greater success and control of the inoculation.
Step 3 - Inoculation
Immediately after sterilization, inject the needle into the inoculation zone marked on the GrainLoving™ .
USE ONLY 4 CC OF GROWING for a 4kg bag.
Cover the injection area with a cross of adhesive tape in order to avoid any entry of contaminants.
Too much liquid culture can cause the grain to become soggy and can actually impede mycelium growth.
Step 4 - Shake
After inoculation, separate the sides of the bag so that the contents are not so compact. Do this step without opening the bag.
Slightly shake the cereal. The objective is to maximize airflow to the mycelium by peeling off the sides of the bag so that the filter is more accessible to the culture.
Step 5 - Incubation
Place GrainLoving™ in a room temperature area out of direct sunlight to begin incubation.
Do not refrigerate.
You may store the liquid culture syringe in a refrigerator for future use (do not refrigerate PLEOROTUS DJAMOR (PNK OYSTER) culture).
Healthy mycelium is white (although in some species such as pink oyster, it will be pink) and will spread throughout sterile cereal or sawdust.
When the bags are completely covered in white, you can consider the spawn colonized and ready to replicate.